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A strong electric field is used for this purpose. In the present manual, protocols for the transformation of about 40 strains of bacteria are described.


Emphasis is placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered. Electrotransformation of Escherichia coll.

Transformation of Bacillus subtilis PB Biotechnology and Food Technology. Clostridium in Biotechnology and Food Technology. Electroporationlnduced Transformation of Clostridium perfringens. Electroporation Induced Transformation of Clostridium beijerinckii. Electrotransformation of Bordetella. Transformation of Campylobacter jejuni.

SlowGrowing Mycobacteria. Electrotransformation of Photobacterium damselae subsp pisddda. Actinobadllus actinomycetemcomitans Electrotransformation of a Periodontopathogen. Frandsella in Medical and Veterinary Applications. Electroporation of the Anaerobic Rumen Bacteria Ruminococcus albus. Electroporation of Legionella Species. Electrotransformation of Lactococcus lactis. Electrotransformation of Salmonella typhimurium. Electroporation of bifidobacteria.

Electrotransformation of Listeria species. Electroporation of Listeria Species with Vectors Greater than 10 kb. Desai , Lee R. View this table: View inline View popup. TABLE 1. Bacterial strains used in this study. Schematic diagram of custom-built generator. Custom cuvette design.

Electrotransformation of Bacteria : Natalie Eynard :

TABLE 2. TABLE 3. TABLE 4. Accepted 7 November Phone: Fax: E-mail: lee. Megehee, and V. Conjugal transfer of transposon Tn into the cellulolytic bacterium Eubacterium cellulosolvens.

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Bayer, E. Chanzy, R. Lamed, and Y. Cellulose, cellulases and cellulosomes. Cato, E. George, and S.

Bibliographic Information

Sneath, N. Mair, M. Sharpe, and J. Holt ed. Chen, C. Boucle, and H.

Knight Laboratory Library

Factors involved in the transformation of previously non-transformable Clostridium perfringens type B. FEMS Microbiol. Dunny, G. Lee, and D. Improved electroporation and cloning system for gram-positive bacteria. Guedon, E. Desvaux, and H. Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering. Holo, H. High-frequency transformation, by electroporation, of Lactococcus lactis subsp. Jennert, K.

Molecular Techniques

Tardiff, D. Young, and M. Microbiology : Klapatch, T. Demain, and L. Restriction endonuclease activity in Clostridium thermocellum and Clostridium thermosaccharolyticum. Electroporation of Clostridium thermosaccharolyticum. Lin, Y. Lynd, L. Weimer, W.

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  • Microbial cellulose utilization: fundamentals and biotechnology. Wyman, and T. Biocommodity engineering. Lynd, R. Grethlein, and R. Fermentation of cellulosic substrates in batch and continuous culture by Clostridium thermocellum. Mai, V. Lorenz, and J. Transformation of Thermoanaerobacterium sp.